CRISPinatoR Tutorial

Filtering Results


sgRNA Filter Panel
Hover over descriptions to highlight information in the image.
  1. When the results for your query are returned, a filter panel will be displayed. Use the dials to select for the features of the sgRNAs you seek.
  2. The filter panel will scroll move with you as you scroll down as long as you have a large enough screen.
  3. Tier - Select the appropriate radio button to display only sgRNAs with the corresponding CRISPinatoR tier.
  4. Exon Number - Use the slider to select the range of overall exon positions you want to see. All other exons will be hidden.
  5. Off‑Target Score - Use the slider to select the minimum Off-Target Score displayed. All sgRNAs with a lower score will be hidden (the font will turn black).
  6. On‑Target Score - Use the slider to select the minimum On-Target Score displayed. All sgRNAs with a lower score will be hidden (the font will turn black).
  7. ESE Distance - Use the slider to select the maximum base-pair distance displayed from the PAM sequence to the closest ESE (upstream or downstream). All sgRNAs with a longer distance will be hidden (the font will turn black).
  8. PAM/CDS Ratio - Use the slider to select the maximum PAM/CDS Ratio displayed. All sgRNAs with a higher ratio will be hidden (the font will turn black). Note: usually 0.05-0.65 is best.